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Nucleic acids, lipids, and carbohydrates Video transcript Remember that when you run a TLC plating lab you have twp phases, the stationary phase shown as this blue silica gel on the plate and a mobile phase. The mobile space is a solvent that's less polar than the solid stationary phase.
Silica gel is very, very polar. Let's say that you had a plate that looked something like this. You had initially spotted two compounds. We'll call them A and compound B. And then what you saw on the plate was that your mobile phase had traveled up to about here, A had traveled to about here, and B had traveled this far.
But what does that really mean? How can we even report these values? The way we'd report them if we were writing up a lab report or writing a manuscript, you'd need something known as the retardation factor, also known as the retention factor or RF for short. RF is equal to the distance traveled by solute over the distance traveled by the solvent.
So the first step you need to do is measure these distances for the different compounds and also for the solvent, also known as the mobile phase. So let's put a ruler next to our TLC plate, much like you would if you were sitting in lab. We'll say that this is 1 unit, 2 units, 3 units, and 4 units.
So we can measure the distance that A has traveled, and that's from the starting line to the center of the spot. And for compound B, again from the starting line to the center of the spot, that's 3 units.
And for the solvent, the starting line to this finish line, that is 4 units. So let's plug that into our equation. If we wanted to solve RF of A, you need the distance traveled by compound A over the distance traveled by the solvent, so let's say A over S.
Here, that would be equal to 2 over 4, and the convention is to report these values as decimal points, so we'll say that this is 0. Now, we'll do the same for compound B.
RF of B is equal to distance traveled by B over distance traveled by S. In this case, that's equal to 3 over 4, or 0. So what can we tell about these two compounds?
If we remember from talking about the mobile phase and stationary phase, compounds that travel really far must be more attracted to the mobile phase, and therefore are less polar.
So we can say that compound B is less polar and travels faster. The opposite is true for compound A.
Since this doesn't move as much, it's more attracted to the polar silica gel, and hence it's more polar than compound B and travels slower. Think about it like it's getting stuck in the stationary phase and doesn't really want to move away from it. So there we've done our first example.
Let's do another one.
In this example, we can see that our initial reaction mixture separated into four different compounds. Let's label these as A through D, with A being the orange spot, B as the yellow one, C as the green one, and D as the purple one.
Again, we'll use the same process that we used earlier. So the first step is to take a ruler and put it next your TLC plate. This is 1 unit, 2 units, 3, 4, 5, and 6. So let's calculate the RF of A.
This is equal to the distance traveled by A over the distance traveled by the solvent, so we need to measure these. First, we can see that A has traveled 1 unit, equal to 1, and the solvent has traveled about 6 units. So we'll say that's 1 over 6 then. Let's convert that to decimals and you have 0.
We can do the same for each these compounds.Separating a Mixture of Biphenyl, Benzhydrol, and Benzophenone by Thin-Layer Chromatography.
Introduction. Thin-layer chromatography (TLC) is a simple and inexpensive analytical technique that can quickly and efficiently separate quantities of less than ten micrograms of material.
Your lab report may look very different than the sample report, but it will contain each of the elements required by CLIA. It may also contain additional items not specifically required but which the lab chooses to include to aid in the timely reporting, delivery, and interpretation of your results.
The separation will be monitored by Thin Layer Chromatography. Fill the column 3/4 full with your eluting solvent. (Please turn in the answers to these questions when you arrive at your NEXT lab.) In a data table, please report your unknown number and what the two components of your binary unkown mixture were from the list of possible.
The write my chromatography lab report sample is transported via the column by the circulation of inert, gaseous mobile phase.
After that, drop the eluant through . The purpose of writing a lab report is to determine how well you performed your experiment, how much you understood about what happened during the experimentation process, and how well you can convey that information in an organized fashion.
Sample Lab Report #2 in the Writing Guidelines for Engineering and Science Students: guidelines to help students of science and engineering make their writing more efficient for others to read and to make the process of writing more efficient for them to perform.